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(In any case, the small wash above the Ingersolls' cabin was unprotected).
Meanwhile, 0.1 mL of elution (from the third wash above) was used in the above procedure.
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Try a hot wash, as above, but focus only on the waistband.
The slides were washed as above, counter stained with DAPI and mounted in vectashield (Vector laboratories).
After washing as above, the bound antibodies were visualized with an ECL detection kit as described previously [44].
Cells were washed as above and αvβ3 integrin binders were isolated using a Becton Dickinson FACSVantage SE instrument (Stanford FACS facility).
The wells were washed as above, and 100 µl of ABTS peroxidase solution (Kirkegaard and Perry Laboratories) was added.
After three washes as above, the cells were resuspended in 2% formaldehyde in PBS.
After washing as above, the bound antibodies were visualized with an ECL detection kit.
The wells were washed as above and then loaded with the solution of recombinant antigen.
After washing as above, the parasites were mounted in Vectashield with DAPI (Vector).
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