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To prepare for experiments using washed solids, whole slurry PCS was washed using a basket centrifuge (Model STM-2000, WeStatesStates, Hamilton, OH, USA) with a nylon filter bag (105 μm pore size, 25% open area, Sefar filtration cloth, Western States) until the glucose concentration in the wash water was below 0.1 g/l.
Following hybridization for at least 17 h, the array was washed using a Gene Expression Wash Buffer kit (Agilent) and scanned in an Agilent Array Scanner.
The pH meter was calibrated before each use using pH 7 and 4.01 buffering solutions and the probe was washed using distilled water between each use to remove any remaining residues.
The pretreated milk (100 L) was applied to a BPG 300/500 column to bind basic proteins, and the column was washed using 0.40 M and 1.0 M NaCl.
Pellet was washed using ultrapure water for three times.
Then the electrode was washed using wash buffer to remove the unimmobilized probe DNA.
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Afterwards, microarrays were washed using Agilent Gene Expression Wash Buffer Kit and scanned with the Agilent DNA microarray scanner G2505B.
GeneChips were washed using the Gene Expression Wash Buffers Pack and scanned using an Agilent DNA Microarray Scanner (G2565CA).
Fine or vintage items should be washed using the delicate cycle, or hand washed in very hot water (wear protective gloves if you're handwashing).
Cells were washed using ice-cold PBSA buffer (1× PBS pH 7.4 supplemented with 0.1% w/v bovine SA fraction V).
Slides were washed using Agilent wash buffers.
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