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The significance of the positive regulation was verified further by the fact that the Per2::luc reporter gene that could not be transactivated by PER1/2 (delta-Per2::luc) lost the phase delay observed in wild-type Per2::luc.
Thereafter, this was verified further in hepG2, HSG, and SHG44 cells, as shown in Figure S2.
The closer relationship between NGMGCTs and ESCs was verified further by hierarchical clustering.
The identity of uPAR and β1 integrin interaction was verified further in mock-transfected HCT116 cells by immunodepleting the cell lysates of β1 integrin or uPAR by five rounds of immunodepletion steps.
These fusion proteins exhibited their appropriate M r (Fig. 3B, left panel) and the latter was verified further by reactivity with L4569; an antibody directed against the CaV2.2 C terminal long-splice region (Khanna et al., 2006b) (Fig. 3B, right panel).
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Differential regulation of histone H2B and NIF-1 were verified further by ELISA (urine, n?=?166) and immunohistochemistry (tissue samples, n?=?32) [179].
However, this notion needs to be verified further.
However this observation needs to be verified further using broader taxon sampling.
These results were verified further in both cell lines using siRNA to specifically target IGF-IR (Additional file 2, Figure S1).
Another, possible way through which the authenticity of the manually curated data could be verified further is by the aggregators and/or various hybrid databases.
The results of the melt curve analysis were verified further by electrophoresis on 1 % agarose gel and photographed on UV light illuminator (Clare Chemical Research).
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