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Most recently, SSAW was utilized to sort individual droplets dispersed in an oil phase.
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For example, HaloTag was employed for investigating the expression and spatial trafficking of integrin in individual cells using a fluorescent HaloTag ligand, and an additional affinity tag was utilized to capture and sort the cells.
Fluorescently activated cell sorting (FACS) analysis was utilized to label CSPCs for a series of putative cartilage stem/progenitor cells markers.
A BD LSR II flow cytometer was used to sort 10,000 single cells per biological replicate, and FlowJo software was utilized to gate for BrdU incorporation.
The non-dominated sorting genetic algorithm II (NSGA-II) was utilized to optimize the thickness of the steel plates based on the approximation model.
Inertial focusing in microfluidics has been widely utilized to sort cells based on cell specific properties for clinical assay, the detection of environmental pathogenic microorganisms and cell biology research.
An elitist nondominated sorting genetic algorithm (NSGA-II) is utilized to perform the optimization, in which the nondominated sorting mechanism is employed to handle the multiple objectives, and the constraint-domination principle is utilized to handle the multiple constraints.
As there is no benchmark in the literature, a popular non-dominated sorting algorithm is utilized to validate the results obtained.
The general methodology of cell-SELEX is very similar to traditional SELEX, but fluorescence-activated cell sorting (FACS) can be utilized to achieve a very high level of separation of MRE bound and unbound cell targets.
These sorts of websites should be utilized to their fullest potential.
Hoechst 33342 dye efflux, another sorting approach in using functional properties of CSCs, are being utilized to isolate side population cells enriched in CSCs.
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