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A barcode scanner was utilized to read the barcode.
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The open circuit voltage (∼±0.8 V) and short circuit current (∼±6 μA) that arise from non zero-crossing current voltage characteristics have been utilized to read the ON and OFF states for non-volatile memory application of the devices.
If the target size is small, then only a small number of contacts between the protein and the DNA can be utilized to read out genomic information and sequence space is consequently minimal.
The MG-RAST v.3.0 online server quality control pipeline was utilized to remove reads of short length and poor quality before annotation and the analysis of metagenomic data [ 26].
Then TopHat was utilized to align these reads to the reference genome permitting four mismatches considering their germplasm variances.
Cross_match (Phil Green, http://www.phrap.org) was utilized to align the reads assigned to each bin to the corresponding ~100 kb of genomic sequence.
Genscan was utilized to identify open reading frame matches to GenBank entries [ 33].
Mothur (http://www.mothur.org/) was utilized to analyze the pyrosequencing reads.
The directionality of the reads was utilized to refine the peak boundaries.
However, considering that the distance of two reads of paired-end was utilized to assemble the sequence by the software, there is an inaccurate distance between two paired-end reads with a length smaller than 90 bp, which may lead to a wrong assembly.
Illumina sequencing was utilized to generate ∼87 million paired-end, 100 nt reads, using RNA from GFL tissue, the same number of reads using RNA from OL tissue, and ∼440 million paired-end, 100 nt reads, using germline DNA.
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