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At this stage, angiotensin converting enzyme inhibitor therapy was initiated; captopril was used, started at 3 mg/d, and increased to 6 mg/d after 15 days.
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For β-actin a DNA control was used starting from 0.14ng (7×108 copies) to 0.14×10−8ng (≤7 copies).
A primer walking strategy was used starting at the central conserved domain.
Then a local refinement was used starting from the alignment candidates close to the optimal solution.
To establish a final regression model, downward stepwise selection was used, starting with a prespecified set of candidate variables.
A grid search was used starting with an initial wide range of values for f, β, and α.
A gradient was used, starting at 95% A for 5 min, changing to 10% A linearly in 10 min.
DTP-IPV-Hib with whole cell pertussis vaccine was used prior to July 1 , 1997and DTP-IPV-Hib with acellular pertussis vaccine was used starting on this date.
A touchdown PCR was used starting at 63° and ending at 50° for a total of 45 cycles using primers listed in Table S1.
A new version of the test will be used starting April 1.
A stepped-care approach has traditionally been used, starting with a single, well-tolerated drug, such as a diuretic.
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