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A highly parallel finite element code was used, running on two supercomputers, solving the transient incompressible Navier Stokes equations on unstructured meshes.
For haplotype analysis, PLINK v 1.06 (http://pngu.mgh.harvard.edu/~purcell/plink/) was used running a sliding window approach with variation of the window size from 2 to 8 included markers and using the option "hap-logistic".
For quantification of gene expression, the 7500 Fast Real-Time PCR system (Applied Biosystems, Foster City, CA) was used running the 'Fast RT-protocoltocol (2 min at 50°C, 2 min at 95°C and 40 cycles of 3 sec at 95°C and 30 sec at 60°C).
To remove repetitive elements in the initial set of cow tRNA gene predictions, RepeatMasker (version open-3.1.8) [ 18] was used running with the following parameters: "-e cross_match", "-slow" and "-species cow".
A staggered training schedule was used, running the mice in cohorts of five or six mice, while alternating the different cohorts through the trials over day 1 and day 2 of the test.
For large scale expression and purification performed by the Desktop II robot (CFS), an H-kit was used, running 6 mls × 6 reaction cups, one construct per cup (six constructs total, NS5B FL, FtsZ FL, and P38α; native and engineered versions of each).
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For protein electrophoresis NuPAGE Novex Bis-Tris gradient gels (4 12%) with 15 wells (Invitrogen) were used (running buffer: MOPS-SDS).
Mr. Garang was killed when the Ugandan helicopter he was using ran into a mountain.
He was used to running his life on his own.
PBS Thermo SCIENTIFICC, #1890535) was used as running buffer.
MSP was used as running buffer for all subsequent experiments.
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