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This reduction was absent when a PrP construct was used lacking its GPI (glycosylphosphatidylinositol) anchor.
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Various control conditions can be used (lacking manipulation or presenting one or more placebo manipulations) and method co-occurrence can be avoided (except, of course, when this co-occurrence is the matter under investigation).
An important limitation of such studies, however, is that all of them pooled data from studies in which different probiotic strains were used, lacking subanalyses to determine effects of individual probiotic strain(s).
Many of the biochemical techniques that might be used lack the resolution to specifically probe individual enzymes and proteins.
Alternatively, residents might be using lack of time as an excuse – either consciously or otherwise – to avoid difficult, seemingly time-intensive conversations with patients and families.
For high-grade AVMs, combined treatments are often used lacking a standard procedure.
An empty pcDNA3.1 plasmid was used in transfections lacking H6PDH to adjust total DNA amounts in all samples.
The same method was used for censored patients lacking data on levels of triglycerides.
A plaque assay was used to confirm lack of viral replication.
A value of P < 0.10 was used to indicate lack of homogeneity (heterogeneity) among effects.
Semiquantitative RT-PCR was used to confirm lack of ftt-2 expression (data not shown).
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