The suspension was centrifuged at 3000 g for 10 min at RT and the supernatant was used immediately for the experiments.
The 6-OHDA in PBS solution was freshly prepared for each experiment and was used immediately for intraperitoneal injections (200 mg/kg).68 Sympathectomy was confirmed in the whole-mount corneas by immunofluorescent staining (described above) to identify sympathetic nerves.
This can be explained in part by differences in study design: in basic studies estrogen treatment was used immediately or shortly after ovariectomy, while in some key clinical trials, estrogen was given years after menopause.
The monomer was used immediately after purification.
Whereas the field notes form was used immediately after the class, to write up research notes.
The cDNA was used immediately or stored at −20 °C until used for the PCR test.
The obtained NP suspension was used immediately for assay or lyophilized for storage at -50°C.
The clear diazonium salt solution thus obtained consisting of the in situ-formed intermediate 5, was used immediately in the coupling reactions.
The same discourse completion task was used immediately before intervention as pre-test, immediately after intervention as post-test, and a period of time after intervention as follow-up test.
OUR values were measured using a 5331 oxygen probe connected to a 5300A oxygen monitor (YSI, Yellow Springs, OH), which was calibrated before each assay according to the manufacturer's instructions and then was used immediately following calibration to ensure accuracy.
