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For the Lrp1btm1wtsi allele, a 359 bp 3' external probe was used (generated by PCR from AB2.2 genomic DNA, using the primers: forward, 5'- AAA AAA TCT TCC TTG AAG GCT CTT GTA AG GTC -3' and reverse, 5'- ATG CAT ATG GAA TGC CAG GGG GAT GTT CAC AC -3') and hybridized with EcoRV-digested DNA to identify restriction fragments of 18.2 kb for the wild-type and a 11.3 kb for the targeted allele.
For the Lrp1btm2wtsi allele, a 500 bp external probe was used (generated by PCR from AB2.2 genomic DNA, using the primers: forward, 5'-GAA AGT GAT CAA ATG AAC ATA TTC AAA TCC TTC-3' and reverse, 5'-CTT GAT CAC AGC TTT CTC TCA ATG GAC TTT AC-3') on BamHI-digested DNA to identify a 10 kb wild-type and a 20 kb targeted allele.
A combination of index terms and text words was used generated by the structured research question.
Data from an early draft of S. ratti genome sequence was used, generated by the Sanger Institute, UK.
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A mixed methodological approach was used, generating both quantitative and qualitative data.
In this work, only distance values from 3D data are used, generating a distance image.
OMEGA v2.4.6 [36] was used to generate conformers.
VISSIM was used to generate the data.
This vector was used to generate pTP03_0882ΔNLS2.
The IMOD package[35] was used for generating the reconstructions.
Antechamber was used to generate parameters for the small molecules.
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