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As substrate, 4-methylumbelliferyl-β-D-N,N'-diacetylchitobioside (Sigma-Aldrich) was used dissolved in sodium acetate buffer pH 5.0 (100 mM) at 2.8 or 3.8 μg.
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-Epicatechin and procyanidin B2 were used dissolved in ethanol.
Two formulations of propofol were used, dissolved in either 10% intralipid (the formulation for clinical use, from Astra-Zeneca, London, UK) or in DMSO (Sigma-Aldrich, Dorset, UK).
Nitromethane is used dissolve the LiCl without oxidizing the aluminum.
When needed, 1% ascorbic acid was used to dissolve ritanserin or 20% PPG (dissolved in bidistilled water) to dissolve DHE.
DMSO (Dimethyl sulfoxide) was used to dissolve the residues of methanol, acetone and chloroform extracts while aqueous extract residues were dissolved in distilled water at different concentrations.
Inks 1 3 were used to dissolve and print precursors of ZIF-8 at required concentrations, and Ink 4 was used to dissolve and print protein and other molecules at required concentrations.
DMSO was used to dissolve the compounds because acetone used in the original method [ 7] did not dissolve all the compounds well.
Control groups were injected with the physiological saline that was used to dissolve the drugs (NaCl 0.9%) or with Tween-80 (Sigma-Aldrich, Madrid, Spain), which was used to dissolve WIN and SR (two drops of Tween dissolve in saline).
Distilled water was used for dissolving S. alata extracts prior to feeding to mice.
Ink 4 was used to dissolve BSA at a concentration of 50 mg/mL.
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