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After washing with TRIS buffer, ABC solution (Vector Laboratories, Eching, Germany) was used applied for 30 min followed by Tyarmid enhancement using the NEN kit (Perkin Elmer, Rodgau, Germany) for 15 min at 1 50 dilution.
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The Rayleigh Ritz method was used applying beam functions as the trial functions.
Pairwise nearest-neighbor matching was used, applying a caliper of 0.05 on a probability scale [ 23].
Cox proportional-hazard modeling was used, applying the forward stepwise analysis method to assess the effects of potential outcome markers.
Scenarios can be used, applied and implemented in a range of ways.
If not sufficient, neutral pH based cleaning liquids may be used, applied according to the manufacturers instructions.
This high potential can only be used applying adequate joining techniques.
For the analysis, the software programs PASW 20.0 and M-plus 6.0 were used applying specific methods.
When single comparisons were made, t-tests were used, applying paired or unpaired analysis as appropriate.
Furthermore, data from the NeoKIP intervention database have been used, applying a case-referent design.
Intravenous clearance can also be used applying only Eqs. 2 and 3 (ref. 37).
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