The dynamics of integron genes in T. denticola indicate that the integrons of T. denticola are fully active and are undergoing active insertion and deletion of cassettes.
Radical amino acid substitutions were defined as those with BLOSUM62 matrix score < 0. The challenge of identifying single nucleotide changes within a sequence that is undergoing frequent insertion-deletion polymorphism has been described [ 48].
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This insertion is in the intron just preceding the large exon encoding the PIGSFEAST repeats that are undergoing concerted evolution [21], [22].
Patients undergoing insertion of a double-lumen CVC in the ICU were randomized to receive either a VCV impregnated with chlorhexidine and silver sulfadiazine, or the standard one.
The first question to address was whether N alkylation is essential, given that most biologically active barbiturates are not 1,3-dialkylated, and free N H groups might be expected to undergo insertion reactions with a rhodium carbenoid.
Besides offering all patients regular meals, they were advised to undergo insertion of a percutaneous gastric feeding tube, unless there were medical contraindications.
Patients had to be suitable and willing to undergo insertion of a portable device and indwelling catheter (Port-a-Cath).
The LiDCO™plus haemodynamic monitor system is suitable for patients who have undergone insertion of arterial and venous catheters (peripheral or central) and require monitoring.
Subjects who had previously undergone insertion of a nasogastric tube were preferred, as stress and anxiety relating to tube insertion may affect gastrointestinal function, which would be less likely in individuals with such experience [ 18].
Adult patients needing a double or triple central venous catheter were sequentially assigned in permuted blocks of five to undergo insertion of a control or RM-impregnated catheter.
