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In general, a large and reproducible decrease in the normalized cell indices occurred ∼15 20 hr post-treatment and was typically completed within 5 hr.
MuTIR primers used in this study included TIR4, TIR6, and TIR8 from Settles, et al. [ 23] as well as the primers 5'-CGCCTCYATTTCGTCGAATCC-3' and 5'-GCCTCCATTTCGTCGAATC-3'. PCR was typically completed with Taq DNA polymerase and buffers from either Invitrogen or Promega with either 5% DMSO or 1 M Betaine added to the final reaction.
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