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Briefly, the Saccharomyces cerevisiae reporter strain AH109 was transformed simultaneously with certain combinations of pGBKT7-based and pGADT7-based fusion constructs using a small scale lithium acetate/single-stranded carrier DNA/polyethylene glycol (LiAc/ss-DNA/PEG) transformation protocol [58].
Similar(59)
Then, from the fifth to the 12th lifting steps, the signals are transformed simultaneously in spatial dimensions y and z, and temporal dimension t using the non-separable 3D structure.
In order to express glucoamylase and α-amylase simultaneously in P. patoris, plasmid ZαAmy was transformed into KM71/9KGla.
It was not possible to transform all four plasmids simultaneously into E. coli strains, so routinely one plasmid was transformed, and competent cells made from these cells to transform the next plasmid, and this process repeated as needed.
But Mick was transformed.
The street was transformed.
Mr. Liao was transformed.
I was transformed.
Petersburg company was transformed.
Even the chef was transformed.
The canyon itself was transformed.
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