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Open-ended interviews were carried out, and the data was transcribed into codes and themes.
After the vote, each senatus consultum and each senatus auctoritas was transcribed into a final document by the presiding magistrate.
Each senatus consultum was transcribed into a document by the presiding magistrate, and then deposited into the building that housed the treasury.
The total RNA was transcribed into cDNA using Superscript III.
Viral RNA was transcribed into cDNA with the Superscript III Reverse Transcriptase Kit (Invitrogen).
RNA of individual patients was transcribed into cDNA, and RT-PCR was performed as described previously [35].
DNase-treated (DNase I, Invitrogen) total RNA (1 2 µg) was transcribed into cDNA using Superscript II reverse transcriptase (Invitrogen) and random primer oligonucleotides (Invitrogen).
One microgram of total RNA from each sample was transcribed into first-strand cDNA using SYBR GreenER two step qRT-PCR kit from ABI Prism (Invitrogen, Carsbad, CA).
For RT-PCR analysis, 2 mg of RNA was transcribed into cDNA with the Reverse Transcription System (Promega, USA) and oligo (dT) primers in a 20-µl volume.
Following phenol/chloroform extraction and ethanol precipitation, the cDNA was transcribed into biotin-labeled cRNA using T7 polymerase (Ambion MEGAScript T7).
RNA was transcribed into cDNA using Omniscript (Qiagen, Hilden, Germany).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com