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The cell medium was thereafter changed at least 5 times/week.
Medium was thereafter changed to RPMI (Mediatech) supplemented with 0.5% pen/strep, 0.5% glutamate and 100 ng/ml activin A (R&D) for one day.
The size was thereafter changed to 1400 × 1024 pixels with a resolution of 72 dpi to create a database that can be accessed through a web interface.
Fractionated total body irradiation (TBI) with high-dose cyclophosphamide was used as a conditioning regimen for HDT until 1995, and was thereafter changed to chemotherapy only (BEAC/BEAM) (Blystad et al, 2001a, 2001b).
P31 and H1299 cells were cultured to about 80% confluence and the medium was thereafter changed to fresh medium containing 0 or 5 mg l 1 cisplatin and/or 0.1 μg l 1 VT-1, and incubation continued for 72 h.
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The statistical significance of the interaction was thereafter calculated from the change in −2 × log likelihood) when the interaction term of vitamin E supplementation and the modifying factor was added to the model.
The statistical significance of the interaction was thereafter calculated from the change in −2 × log (likelihood) when the interaction term for vitamin E supplementation and the modifying factor were added to the model.
After two weeks, culture media were replaced with fresh ones and thereafter changed every 7 days.
Medium was not changed thereafter.
Thereafter, medium was changed and cells were incubated for another 20 min.
Discard of non-adherent cells and first medium change were performed after 2 days: thereafter medium was changed three times a week.
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