First, insecticides are tested singly, despite the European regulator reporting that "pesticides are often applied in tank mixes (two to nine active ingredients at the same time)".
Sulfadoxine and pyrimethamine were tested singly and in combination with six of the field isolates at fixed ratios of 1∶1, 1∶3, 3∶1, 1∶4, 4∶1, and 1∶5.
Parents were tested singly in a sound-attenuated recording box (70 cm×50 cm and 50 cm) which was equipped with a small metal wire cage containing a single perch, a microphone (C2, Behringer GmbH, Willich, Germany) approximately 20 cm from the perch and a small loudspeaker (V20, Logitech, Morges, Switzerland) next to the microphone.
The variables were tested singly and also as groups of confounders.
Importantly, for safety of the therapeutic, all candidate T cell epitope peptides must finally be tested singly and in combination to ensure lack of ability to bind and cross-link inflammatory cell-bound IgE.
The drugs were tested singly at scalar concentrations of 0.001, 0.01, 0.1 and 1 μg/ml for Pacl and Dox, and 0.01, 0.1, 1 and 10 μg/ml for 5-FU.
A concept of realizing a standard for aerosol particle number concentration was tested, based on generating singly charged aerosol particles in the size range from 10 up to 500 nm.
When membrane insertion was tested, both unmodified and singly PEGylated forms of Sec61βOPGS77C,L79C were visibly N-glycosylated, in agreement with our previous results.
Primary antibodies to be tested were added (singly, diluted in 20 μl of blocking solution), and the resulting mixtures were each covered by a cover slip and incubated overnight at 4 °C.
