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Differential item functioning (DIF): DIF was tested in reference to age, gender and education.
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The specificity of the real-time PCR assay developed for the identification of M. yongonenese was tested in 28 reference strains of mycobacteria via Cq and melting curve analyses.
One of these beams was tested in its virgin condition to serve as reference, while the remaining five beams were tested after being strengthened using carbon fibre wrap, strip or grids.
Performance and accuracy of the system was tested in the laboratory using five reference soil samples with different textures.
Each clinical isolate was tested in duplicate along with the reference E. histolytica strain (HM1: IMSS).
The expression stability of eight candidate reference genes was tested in leaves, roots and callus.
The combination for each reference strain was tested in triplicates.
It is important to note that the experiments are independent of each other, and different measurement systems had to be tested in each case to find a suitable reference standard, selecting the appropriate deuterated solvent and elaborating the appropriate acquisition parameters, e.g., relaxation times (see Fig. 4).
The list of specific fragments of 25 60 bp was reviewed and the potential candidate probes were tested in silico against reference databases (RDP, NCBI) with the aim to verify their specificity.
It has been suggested that different reference genes should be tested in each study setup to find the most suitable one not influenced by the experimental treatment [ 39].
All potential candidate probes were tested in silico against reference databases (RDP, NCBI) for their specificity.
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