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To adapt the biosensor to the work with real samples, its sensitivity to urea was tested depending on the concentration of the working buffer and the salt and protein concentration in the samples.
The CIV protocol was developed based on recommendations of IDSA for the loading dose (30 mg/kg), but a new protocol for the maintenance dose was tested (depending on weight and daily Creat Cl).
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Three different antibiotic concentrations were tested depending on the antibiotic used and bacterial strain employed.
Several statistical distributions were tested, depending on whether the indicator was a continuous or a discrete variable.
15 Differences among the groups were tested depending on data types.
In total between 691 1,321 isolates were tested depending on the year and number of countries submitting date for that year (Table 1).
Thus, for each liner, at least four specimens were tested (depending on material availability), which led to a total of 889 small punch tests performed on the 185 retrievals.
However, because it is an "in-house" method, it is possible to customize the assay by adding or deleting genes to be tested depending on the particular resistance mechanisms of interest.
Seven concentrations of each of the CSC triplicates, ranging from 3 to 250 μg TPM per plate, were tested depending on the potency of the sample on a given Salmonella strain (i.e., three brands, three replicate CSC per brand, tested at seven concentrations each alongside solvent control).
The doctor may recommend both partners be tested depending on each person's medical history.
The minimum number of batches that should be tested depends on the variability of the reference product and on the assay variability.
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