Sentence examples for was terminated using from inspiring English sources

The reaction was terminated using 0.5 M EDTA.

After digestion, the enzymatic reaction was terminated using 5% acetic acid.

The reaction was terminated using 20 µl of 0.4 M hydrocholoric acid and 1 µl of 330 mM EDTA.

The reaction was terminated using 2N H2SO4 and the absorbance was measured at 492 nm in an ELISA reader.

Briefly, RKIP (5 µg) was combined with increasing concentrations of locostatin or DHPE and the reaction was initiated with 100 mM ATP containing 5 µCi of [γ-32P]ATP at 30°C for 10 min. The reaction was terminated using sample buffer (6×) and boiling at 100°C for 5 min. Lysates were separated on SDS-PAGE (12%), transferred to nitrocellulose and visualized by exposing to film.

One or 2.5 µg of RNA was diluted to a total volume of 8 µL and was treated with 1 µL of RQ1 RNase-free DNase (Promega, Madison, WI) and 1 µL of DNase buffer at 37°C in a thermal cycler (Techne, Model TC-312, UK) for 30 min. The reaction was terminated using 1 µL of stop solution and incubating the samples at 65°C for 15 minutes.

The polymers were terminated using amine-functionalised glass coverslips, enabling the synthetic procedure to be reproducible and scaleable.

All reactions were terminated using 1 µl of 1 M formic acid.

Reactions were terminated using 50 μl of stop solution.

All MMP cleavages were terminated using GM6001 (Sigma-Aldrich) and all cathepsin and aggrecanase cleavages using E64 (Sigma-Aldrich).

Enzymatic reactions were terminated using 100 μl of 0.5 m sulphuric acid and the absorbance read at 450 nm using the SkanIt plate reader (Thermo Electron).