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Each positive slide was systematically confirmed by all three senior microscopists.
First, the synchronization of experimental cultures by age and growth stage was systematically confirmed by protein quantification and fluorescence microscopy.
Species identification was systematically confirmed by sequencing the rrs gene.
For all of the assays, when an amplified product was detected, T. whipplei was systematically confirmed by sequencing, as previously reported [ 19].
If a first PCR assay was positive, the result was systematically confirmed by a second PCR assay using a second set of primer pairs.
The identification was systematically confirmed by manual interpretation of the MS/MS spectra by the "peptide sequence tag" method using the BioAnalyst and PeptideSearch softwares (www.narrador.emblheidelberg.de) (20, 21).
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The global tendency for ChIP-Seq data to yield motifs for the ChIP'd TF proximal to the peakMax is systematically confirmed here with a study of ~340 ChIP-Seq datasets for ~95 TFs (human and mouse).
Presumably, these policy changes have significantly contributed to a reduction in malaria burden and hospital admissions; however, caution should be exercised before generalizing these trends to the whole country because these findings have not been systematically confirmed in remote rural provinces of Gabon.
The morbidity of malaria has steady declined in the urban regions of Gabon between 2000 and 2008, but caution should be exercised before generalizing this trend to the whole country because this finding has not been systematically confirmed in remote rural provinces.
On the assumption that brain lesions are transiently associated with decreased extracellular Mg2+ concentration, even if this observation has not been systematically confirmed in humans, Mg2+ solution infusion was tested and was found to worsen the outcome in patients with traumatic brain lesions.
In one large multi-country study, body temperature following adjunct PPH treatment with 600mcg of sublingual misoprostol was systematically measured, confirming a rate of high fever of 7% (48/704) [ 13].
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