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After 3 days, 0.5 µCi/well 3H-thymidine was supplemented for 18 h.
This is in accordance with the data on insulin resistance in obese men with metabolic syndrome.18 In that study, CLA was supplemented for 12 weeks to 19 men without significant changes in M, fasting insulin, fasting glucose, HbA1c or leptin, whereas the pure CLA isomer trans-10, cis-12 caused a significant decrease in M (14%) and an increase in fasting glucose (3.8%).
Excess substrate was supplemented for NOB and the percentage of NOB in the nitrifying community in the flocs increased.
To acclimatize the strain, 2%% glucose was supplemented for suitable growth of the strain in growth medium comprising substrate phenol and cyanide.
For calcium studies, cells were incubated with the appropriate compounds in PBS and 1.2 mM CaCl2 was supplemented for the calcium-positive samples.
For osteogenic differentiation, media was supplemented for 2 weeks with 50 µM Ascorbic acid, 0.1 µM Dexamethasone, and 10 nM beta-Glycerolphosphate (Sigma-Aldrich, St . Louis MO) and staining with Alizarin Red S, per the manufacturer's protocol (Sigma-Aldrich, St . Louis MO).
Similar(39)
Sumbal is most unlikely to be supplemented for next week's Derby, his French-based trainer said.
Toast Of New York, the easy winner of the UAE Derby at Meydan for Jamie Osborne and Jamie Spencer, will be supplemented for the Derby at Epsom on 7 June.
These have been supplemented for us by Hisham Matar, the distinguished Anglo-Libyan novelist whose father was "disappeared" by Gaddafi more than two decades ago.
"I'd say there is a strong possibility he'll be supplemented for the Irish Derby, if all is well between now and then.
Rats, age 28 days were supplemented for 4 weeks with high Ca (1.2%), adequate Ca (0.5%) or low Ca level (0.2%).
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