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GADD45A was successfully sequenced for 38 samples (17 normal epithelium samples and 21 tumor samples).
Subsequently, β-globin was successfully sequenced for 166 individuals in total (some individuals could not be resolved owing to the presence of heterozygosity for multiple length variants).
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Twenty (of 23) Caspian tiger museum samples (Table S1) were successfully sequenced for at least one segment of five mitochondrial genes – ND5, ND6, CytB, ND2, and COI (1257 bp), amplified as eight short amplicons to facilitate PCR of ancient material (see Methods).
PCR products were successfully sequenced for all KC patients.
At least one clonal Ig gene rearrangement could be successfully sequenced for each patient.
A total of 70 L. sinapis samples were successfully sequenced for this marker.
For the NGS dataset, 387 loci were successfully sequenced for an average of 549,304 bp per sample before trimming.
Of the 63 NoV isolates, 43 (68.3%) were successfully sequenced for phylogenetic analysis, including 7 GI and 36 GII isolates.
A total of 146 individuals were successfully sequenced for an 850 base pair DNA fragment of the mitochondrial ND2 gene.
A+T-rich regions were successfully sequenced for species, S. agrili, D. longicaudata, C. vestalis and M. camphoraphilus.
Full mt genomes were successfully sequenced for the vermetids, Dendropoma gregarium, D. maximum, Eualetes tulipa, and Thylacodes squamigerus.
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