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This subtractive hybridization was performed using sample cDNAs (tester) from inoculated plants, which was subtracted with cDNAs (driver) from non-inoculated plants.
Briefly, the background was subtracted with rolling radius of 50 pixels under light background selection and the color of the image was inverted.
After the experiment the background of each microscopic field was subtracted with the Subtract Background method of the ImageJ software using a rolling ball radius of 300.
The background of each microscopic field was subtracted with the Subtract Background method of the ImageJ [45] software using a rolling ball radius of 300 [46].
After a first round of sequencing, the library was subtracted with the 8736 already-sequenced clones.
Plate absorbance was measured at 450 nm, and background was subtracted with readings at 570 nm.
Similar(47)
The two background data sets are added and scaled to the source data when being subtracted with the Sherpa subtract command, or modeled with set_bkg_model.
The signal and background training samples used for the BDT are derived from the Λb0 → pK−π+π− control sample, since its kinematics and topology are similar to the decays under study; background in this sample is subtracted with the sPlot technique41, a statistical technique to disentangle signal and background contributions.
The Cerenkov is subtracted with the result that the scintillation signal is left behind.
After the original Nth frame is shifting, the predicted N + 1th frame is subtracted with original N + 1th frame of the video sequence.
Mean values and s.d. for each concentration at each time point per plate were subtracted with the reference value (no treatment) and plotted for each cell line.
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