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Background activity of sample control without D-galacturonic acid was substracted from sample measurements with D-galacturonic acid.
The average OD of the reaction mixture in the absence of compost was substracted from the OD of mixtures containing fungal colonized compost or pure mycelium.
Background was substracted from raw data and infected cells were normalized to 100%.
The remaining fluorescence in the bleached region after bleach was substracted from all data points.
This offset was substracted from the potential field in order to be able to compare the different configurations.
The mean RLU value of the negative control was substracted from the mean RLU value of each test sample.
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Essentially, this approach represents an in silico substraction procedure, where the proteomes of the non-mycorrhizal species are substracted from the proteomes of the AM-competent species to yield a fraction of the proteome that is expected to be enriched in AM-related proteins.
The measurements were done once, and blank values were substracted from all readings.
The signal intensities from the extracellular sample were substracted from the corresponding total stained sample to determine the fluorescence signal that derived from intracellular chlamydiae.
Nonspecific background densities were measured at each brain level in a region devoid of MBP immunostaining and were substracted from the cingulum values.
The value from these controls (2.54±0.31 DPM per 103 cells) has been substracted from the values corresponding to Figures 1, 2, 3 and 4 and Supporting Figure S2.
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