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Their use was subsequently restricted in several other countries.
For the P comI -GFP fusion the promotor region of comI was amplified using the primer pair comI13f_KpnI/comI13r_ClaI. The PCR product was subsequently restricted with ClaI and KpnI and ligated into the likewise restricted vector pMUTIN-GFP+, resulting in plasmid pMUTIN-comI.
Analysis was subsequently restricted to the remaining 23,496 CpG loci (12,956 genes), 18,742 located within CpG islands and 4,754 located outside of these regions.
The study population was subsequently restricted to the 73.5% of patients who, according to the GP, had attended general practice as part of the cancer diagnosis.
The co-expression network was subsequently restricted to genes annotated as transcription factors (TFs) in the Animal Transcription Factor Database (ATFDB, [ http://www.bioguo.org/AnimalTFDB/index.php]).
After the ventricular injury, raldh2 was activated in the endocardium, but its expression was subsequently restricted in endocardial cells at the injury site one day post-trauma as the repairing cardiogenesis began.
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De Bruyne's starts were subsequently restricted to three League Cup games, and in the last week of December he told the club that he wanted to leave.
Population allocation in LUISA is subsequently restricted by three factors.
Savage [14] proposed a pre-Oligocene divergence between these genera on the Asian mainland, but that adaptation to tropical habitats led Barbourula to be subsequently restricted to the Sunda Shelf.
Atonal expression is subsequently restricted to one cell per cluster, which becomes the R8 photoreceptor.
Indistinguishable or closely related strains were subsequently restricted with ApaI for clustering confirmation.
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