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Further confounding factors arise as recent studies have revealed the presence of the C-epimer form of 25OHD3 in adult blood samples [11], which was subsequently quantified in further studies [3, 12, 13].
NHS ester of succinic monoester or carbonate of mPEG of 5,000 Da was synthesized and reacted with excessive ethanolamine in dimethylformamide at 25°C for 15 min. Residual ethanolamine was subsequently quantified by absorbance at 420 nm after reaction with 2,4,6-trinitrobenzenesulfonic acid (TNBS) at pH 9.2 for 15 min at 55°C followed by cooling with tap water.
The cDNA was subsequently quantified via spectrophotometry.
Chemiluminescence was subsequently quantified using ImageGauge software (FujiFilm).
The percentage of cells positive for FA was subsequently quantified, as already described [17].
The degree of effectiveness was subsequently quantified by fresh weight and chlorophyll concentration.
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Fragments were subsequently quantified by automated QIAxcel agarose gel electrophoresis (Qiagen).
The target analytes were subsequently quantified using inductively coupled plasma optical emission spectrometer (ICP OES).
Sections were stained with a commercially available antibody to 5MC and immunocytochemistry levels were subsequently quantified using a computer-assisted true-color imaging system.
The fungistatic (inhibitory) and fungicidal (lethal) effects of Eh and Nv volatile-rich atmospheres were subsequently quantified in Petri dishes with either PDA or PDA medium amended with carbendazim, which allowed germination rates to be determined at 18 and 48 h post-inoculation, respectively.
Activities of these proteins were subsequently quantified by densitometric analyses, with the control set to 100%.
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