Sentence examples for was subsequently fixed from inspiring English sources

The filtered mucin was subsequently fixed with 4% paraformaldehyde (prepared in PBS at pH 7.4) (Sigma-Aldrich, MO, USA) for 5 min, followed by careful rinsing with Hanks' solution twice.

Each specimen was subsequently fixed using six implants and was successively measured for the load.

One #3 gland was subsequently fixed overnight in 4% buffered formaldehyde (ICM Pharma, Singapore) for paraffin embedding.

The DNA was subsequently fixed by successive heat (80°C during 2 hours) and UV (120000 μJ) treatments.

The brains were then embedded in quail egg yolk, which was subsequently fixed with 4% paraformaldehyde over six days.

The agar was subsequently fixed by 2.5% glutaraldehyde in PBS at 4°C for at least 2 h.

Each specimen was subsequently fixed in 4% paraformaldehyde (pH 7.4) for an additional 30 min then stored at −4°C.

The liver tissue which was dissected out was subsequently fixed in the 10% formalin, dehydrated in gradual ethanol (50-100%), cleared in xylene and embedded in paraffin wax.

The PGA sheet was subsequently fixed on the visceral peritoneum, and, after fixation, 0.9 mL of physiological saline solution was sprayed over the PGA sheet.

Following this, eluted protein was separated by SDS/PAGE and the gel was subsequently fixed and stained with CBB (Coomassie Brilliant Blue) (R250) to detect protein and then used for MS analysis (as described below).

The jig-plate assembly was subsequently fixed to the craniolateral surface of the femur by pulling the sutures through under the femur, allowing the assembly to be tightened to the femur.