Primary antibody detection was performed with anti-rabbit or anti-guinea pig and alkaline phosphatase-conjugated secondary antibodies (Jackson Immunoresearch), was subsequently detected with Dako REAL Detection System Chromogen Red (Dako), and was counterstained with Mayers hematoxylin (Sigma).
A four-fold increase in the number of precursor fat cells was subsequently detected in the skin surrounding the dormant follicles.
Regarding yeast succession, W. anomalus disappeared from the community, while K. unispora was subsequently detected.
The activity and specificity of the magnetic gold nanoparticle-coupled HPA mAb probes was subsequently detected using laser confocal microscopy, flow cytometry, and atomic force microscopy.
Exogenous gene expression was subsequently detected at multiple locations that were distant from the injection site but within the confines of the bleb created by the intradermal bolus.
Since this specimen contained a large proportion of whole blood, the extract was retreated with the ion-exchange mixed bed resin, and a second round of amplification was performed and bacterial derived PCR product was subsequently detected.
β2GPI bound to cardiolipin was subsequently detected with anti-β2GPI antibodies.
It was subsequently detected in humans, (primarily in males), in sweat, urine, plasma and saliva [13].
For two other subjects, influenza virus was not detected in the nasopharyngeal swabs by qPCR or rapid test, but was subsequently detected in their cough aerosols by qPCR.
The eluted Aβ was subsequently detected by an Aβ42-specific ELISA, utilizing an Aβ x-42 specific capture antibody (12F4, Covance, Princeton, NJ) and HRP-conjugated 4G8 antibody (Covance) for detection.
This fish was subsequently detected on the Lippy Point Line off the Northwest tip of Vancouver Island, so northward migration over the continental shelf continued after exiting the Strait of Juan de Fuca.
