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Each barrel was subsequently connected to separate channels of a pressure-driven flow controller (Fluigent, France).
The trap was subsequently connected in series to the separation column, and the whole columns were developed for 100 min with a linear acetonitrile concentration gradient made from 5 to 35% solvent B (10% distilled water and 90% acetonitrile containing 0.1% formic acid) at the flow-rate of 300 nL/min.
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Some Tox1 scaffolds (597/3 L8 into OXI1/TOX9) were subsequently connected by targeted sequencing.
Based on the largest covariance suggested by the MIs, two error terms were subsequently connected with two-headed curved arrows.
A tracheostomy tube was then inserted and secured in the trachea, and the animals were subsequently connected to the IPML apparatus as described by Von Bethmann et al. [ 22].
The method is based on an isolation-with-gene-flow model, where a single population back in time splits into two, which are subsequently connected by some gene flow.
The method assumes an isolation-with-gene-flow model, where a single population at some point back in time splits into two, which are subsequently connected by some level of gene flow.
These central data were subsequently connected to several third-party annotations, including gene and protein features (RefSeq), taxonomic information (NCBI Taxonomy database), gene ontology (GO), functional classification (Swiss-Prot/TrEMBL), domain and protein family classification (Pfam) and enzymatic activity (KEGG).
In addition, 2 stainless acupuncture needles were inserted into the muscle layer of the bilateral Zusanli (ST36) and the Shangjuxu (ST37) points (which are similar to those of a human), which were subsequently connected to an EA machine (Trio 300, Japan).
A new street (present-day Avenue Winston-Churchill) connecting the Champs-Élysées to the bridge also was subsequently built.
He was subsequently fired.
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