Sentence examples for was subsequently concentrated from inspiring English sources

PolyA RNA was subsequently concentrated by ammonium acetate ethanol precipitation and concentrations were determined using the Qubit fluorometer (Invitrogen, Carlsbad, CA) and the Quant-iT RNA Assay Kit (Invitrogen, cat#Q33140).

The Kozan Fault Zone initially occupied a wide zone of distributed strike-slip translation in the Early Pliocene, but was subsequently concentrated into a narrowly focused strike-slip fault zone in the Late Pliocene and Quaternary.

The virus stock was subsequently concentrated and purified by ultracentrifugation (228 000 g, 2 h, 4°C).

The filtered supernatant was subsequently concentrated using 3 kDa cut-off filters (Merck Millipore, Billerica, MA).

The paddle chimera-β2 complex thus purified was subsequently concentrated to 8 9 mg/ml (Centricon-100; Millipore, Billerica, MA).

M13 was subsequently concentrated approximately tenfold by utilizing the ATPMS, which led to a tenfold improvement in the LFA detection limit to 5 × 10 pfu/mL.

The fractions containing proteins (MBP-ΔN597 or MBP-ΔNCP69) were confirmed by SDS PAGE, and were subsequently concentrated using 10 kDa MWCO Vivaspin Sample Concentrators (Sartorius Stedim Biotech).

Purified DBLs were subsequently concentrated by means of Macro- and Micro-sep concentrators.

S1 and F1-F10 were subsequently concentrated to ∼20 ul using a speed-vac (Savant), and analyzed for protease activity by gliadin zymography.

The samples were subsequently concentrated using Ultrafree-0.5 (5-kDa cut-off) centrifugal filter devices (Millipore, Billerica, MA, USA) and final protein concentrations were determined from the absorbance at 280 nm by using sequence-derived extinction coefficients.

Viruses were subsequently concentrated from conditioned media harvested 48 and 72 h after transfection by ultracentrifugation at 25,000 rpm in a Beckman SW28 swinging bucket rotor.