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In addition, as pointed out by Duret and Hurst [ 33], non-coding DNA is subject to insertion of AT rich transposeable elements.
Kinetoplastid mitochondrial mRNAs are subject to insertions and deletions of uridylate residues, sometimes >100 such insertions/deletions per transcript [ 16].
To prevent ascertainment bias, every cercopithecine species in this study was subject to this methodology and Alu insertion loci were identified from all species.
The intergenic regions between these three genes are hypervariable (as indicated by the variety of colors in Figure 9) and have been subject to multiple insertion and deletion events.
Due to the particular mutation mechanism acting on these repetitive sequences by replication slippage [ 35, 36], microsatellites are subject to frequent insertions and deletions.
It is a systematic process to ensure that a database structure will not be subject to anomalies after insertion, update, and deletion, that could lead to a loss of data integrity (34).
Then, each organism will be used to create a population of 10,000 of its copies and each population will be subject to random gene insertions so that each organism receives one extra gene.
Each resolved clone was subjected to DNA sequence analysis to confirm the in-frame insertion.
The resulting vector was subjected to in vitro mutagenesis (EPICENTRE PCRCR and DNA sequencing verified insertion of Tn5 at nucleotide 722 of nmb76.
Genomic DNA isolated from individual clones was subjected to plasmid rescue for obtaining chromosomal DNA flanking the transposon insertion sites.
Each F1 animal carrying a transgene was picked and genomic DNA from its descendants was subjected to a heteroduplex mobility assay42 to detect the presence of short insertions or deletions in the ttr-11 or ttr-57 gene.
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