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In combined experiments where both the somatic and synaptic currents were recorded, first LI mEC was stimulated with a stimulation electrode thus evoking a synaptic response and after 200 300 ms, a laser pulse was flashed to uncage MNI-glutamate evoking somatic current.
Continuing to hold at -45mV, cells were then subjected to synaptic stimulation protocols, in which the optic chiasm (OCh) was stimulated with a bipolar stimulating electrode (FHC, Bowdoin, ME) to activate retinal ganglion cell axons.
One group was stimulated with a mild current through the connection and the other was not.
Afterwards, each volunteer was stimulated with a 1.6 milliamp current, or assigned to a control group that received the same procedure with the electricity switched off.
Even Dickens would have struggled with Michael Mussa -- balding, bespectacled and a lifelong bachelor," who "tended to stand nervously sipping a drink at social gatherings, unless he was stimulated with a remark or question about economics".
Similar effects were observed when microglia was stimulated with a second TLR agonist, LPS.
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Cells were stimulated with a-factor for 2.5 3 h.
In multiple cell recordings, each cell was stimulated with an interval of 60 s in an alternating manner.
Activation of SF38A-K23 deacetylation was stimulated with an EC50 of 22±16 μM, comparable to the potency of resveratrol against Sirt1 in the FdL assay (EC50~50-100 μM) [ 3].
After a 15-min equilibration, the diaphragm strips were stimulated with a S88X Pulse Stimulator (Grass Technologies, Warwick, RI, USA) and platinum wire electrodes (Radnotti, Monrovia, CA, USA).
Although the PC6 acupoint can be stimulated with a variety of methods (acu-stimulation device, acupressure, acupuncture, capsicum plaster), the important concept is stimulation of the correct acupoint.
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