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One week after the last infusion, electrodes were positioned on the neck over the vagus nerve and the animal was stimulated for 1min.
As hazardous products occur and may generate biocompatibility or environment problems, a recent development of 'green synthesis' was stimulated, for which environmentally friendly reducing agents are used, including saccharides or natural extracts [8].
Prior to RNA isolation, one cell line was stimulated for 24 h with 5.0 ng/mL of IL-1α,5.0 ng/mL of TNF-α, and 25 ng/mL of IFN-γ.
Sixty minutes later, the mammary gland of each cow was stimulated for 5 min. Blood samples were collected from 20 min before to 120 min after the start of the treatment.
The eye was stimulated for 24 seconds while collecting spatially binned (2×2) frames of the eye at 4 Hz.
For induction of ROS, whole blood was stimulated for 30 minutes with LPS, P. aeruginosa and S. aureus.
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For experiments that used the inactivated dengue antigen, splenocytes were stimulated for 18 hrs while peptide stimulations were performed for 6 hrs.
Acupuncturists say there are 365 pressure points that must be stimulated for the therapy to heal.
When new roads are built in sub-Saharan Africa, for example, demand is stimulated for cars, trucks and everything they carry -- all of which could be supplied by American businesses.
Briefly, NB4PMN cells were stimulated for 1 min at 37 °C with or without 1 μM fMLP.
Methods: In this modified protocol, cells are stimulated for a cAMP response in standard media/buffers, washed and then lysed.
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