Complementary technologies include cell lysate microarrays, in which the protein repertoire of a number of cell populations are spotted in parallel on a slide and assayed for reporters of cellular state [18], [19], although all data are population averages rather than per-cell readouts.
All strains were spotted in parallel on plates of identical medium.
To explore further, other proteins (e.g., bovine serum albumin, ovalbumin and casein) were spotted in parallel as controls.
Cells lacking LRS4 or CSM1 were spotted in parallel with WT cells but were photographed later because the two types of mutant cells grow more slowly than WT cells [ 52].
Each probe was spotted in three replicates.
The oligonucleotides were spotted in an "array-of-arrays" configuration that facilitates analysis of 80 individual samples in parallel on each microscope slide [ 24].
These probes were spotted in triplicates.
All samples were spotted in duplicate.
Genes are spotted in quadruplicate.
BACs were spotted in quadruplicate.
