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SMB was specifically highly expressed in callus and lateral root cap rather than secondary cell wall enriching tissues, suggesting a specific role in cell division and root development (Additional file 4).
The gene encoding for the MFS permease (Trire2_69957) that was specifically highly upregulated in cellulose may be involved in the transport of disaccharides, due to a high similarity with a putative maltose permease of the human pathogenic fungus Talaromyces marneffei[ 24].
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For example, OsYSL15 is specifically highly expressed in rhizomes, suggesting a specific role in root development.
Therefore, the novelty of our approach consist in achieving efficient molecular classification of CaP with regard to final pathological diagnosis without using genes that are specifically highly up- or down-regulated in benign versus cancer tissue.
We searched public microarray measurements for genes that were specifically highly expressed in PaC.
In this study, IGF2BP3 was found to be specifically highly expressed in human placental cytotrophoblast cells and TC.
The expression analysis for 20 of the 53 genes identified 15 genes that were specifically highly in mammary gland and may contribute to milk production traits.
A total of 105 probesets (corresponding to 89 genes) were found highly expressed in T cells at the time of transduction, while 120 probesets (relative to 113 genes) were specifically highly expressed in HSCs (Fig 3 heatmap and Supporting Information 'Differentially expressed genes in T cells vs. HSC').
This result is based on our observations that (1) WDR66 is specifically highly expressed in esophageal squamous cell carcinoma and high WDR66 expression correlates with poor overall survival, (2) WDregulatesates vimentin and occludin expression and plays a crucial role for EMT, and (3) knockdown of WDR66 suppresses cell growth and motility and decreases cell viability of ESCC cells.
For examples, an alternative exon in CTNND1 is specifically highly included in R-NSCP1 (Fig. 3A), an alternative exon in CDCA7 shows a high inclusion level only in R-NSCs at P1 and P6 (Fig. 3B), an alternative exon in Abl-interactor 2 (ABI2) shows nearly exclusive inclusion in R-NSCs (Fig. 3C), and an alternative exon in ARFIP1 shows R-NSC-specific low inclusion (Fig. 3D).
A previous study showed that this gene was specifically and highly expressed in testis, indicated that this gene may play important roles in reproduction [ 69].
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