Trastuzumab (Herceptin™), a humanized antibody specific for HER2/neu, was specifically detected compared to an equal amount of a control IgG employing our method (Figure 1, D).
The specific PCR band was specifically detected only in the anti-HA immunoprecipitated chromatin, when compared with anti-IgG immunoprecipitated control chromatin.
An overexpressed FAF1 protein from the pJG4-5/FAF1-HA.tag construct in yeast cells was specifically detected using an anti-HA specific antibody with GST-CD40wt and A mutant but not with the CD40mT6 or CD40AmT6 or the LMP1 control, indicating the requirement of the TRAF6-binding domain of CD40 for its interaction with FAF1.
ER was specifically detected on cells expressing paramyosin, a specific helminth cell marker.
Mitochondrial protein COX IV was specifically detected in isolated mitochondrial sample and this sample lacked any detectable contamination of abundant cytosolic (LDH), nuclear (PCNA), and endoplasmic reticulum proteins (GRP78), except for detectable contamination of catalase from peroxisome and cathepsin D from lysosome.
GST-FoxO1 DBD phosphorylated by purified aPKCλ in vitro was specifically detected by anti-pSer218 antinodies in a dose dependent manner (Supplementary Fig. 3c).
Viral dsRNA from each of the serotypes was specifically detected after reverse transcription, real-time PCR and melting curve analysis.
In case of E. coli cells, an increase in the emission intensity ratio was specifically detected after exposure to vitamin B12.
When we applied 5-ethynyl-2′-deoxyuridine (EdU 20 to the cells to visualize DNA synthesis, the signal was specifically detected in cells with tip growth and cell division in all cases out of 21 cell pairs (Fig. 4a).
The concentration of l-lactic acid was specifically detected by SBA-40E biosensor analyzer (Shandong Province Academy of Sciences, China).
And β-1,3-1,4-glucanase β-1,3-1,4-glucanase β-1,3-1,4-glucanase β-1,3-1,4-glucanaseroteins from Bacillus sp. GFP-2 culture supernatant wase analyzed by specifically western blot (Fig. 4E, lane 1), further confirming that Bacillus sp. GFP-2 indetectedld express β-1,3-1,4-glucanase, which may, at least partially, contribythiso its antibodyobial activities.
