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ERα was specifically depleted using shRNA via retroviral transduction.
Conversely, nucleolin was specifically depleted by the anti-nucleolin antibodies, while the levels of Ro60 and La were unaffected (Fig. 3B).
Similar observations were made when L1 was specifically depleted using siRNA [ 26].
Proteomic and biochemical analyses indicated a particular set of proteins involved in motility was specifically depleted in the dyf13 mutant.
To understand the functional importance of NPM in the nucleolus, endogenous NPM was specifically depleted by RNAi in HeLa cells.
The study revealed a significant reduction of swelling during the development of AIA in SNS-gp130−/− mice in which gp130 signaling was specifically depleted in sensory neurons.
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These three highly abundant lncRNAs could be specifically depleted by adding corresponding specific probes in the RiboZero protocol, thus improving the sequencing depth of the remaining RNAs.
This indicates that NK cells, but not NKT or T cell populations, were specifically depleted [41] [43].
For example, while the contents of 18∶3-SE were specifically depleted in CaCdr1p overexpressing pairs, they were higher in CaMdr1p over-expressing pairs (Figure 3A).
As expected, CD11b+ macrophages (F4/80+) were specifically depleted in the peripheral blood and in the metastasis-bearing lungs (Figure 4C and Figure S7C).
We therefore investigated the role of langerin+ CD8α+ DCs after injection of antigen-loaded DCs in a transgenic animal model in which langerin+ cells can be specifically depleted in vivo.
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