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H2O2 (30%, 7.5 mL) was slowly added, with continuous stirring, for 30 min at ambient temperature.
Dichloromethane (DCM; 25 ml) was slowly added to TFB (100 mg, 0.62 mmol) in a sample vial at room temperature.
8-hydroxyquinoline solution was slowly added to aluminium nitrate solution.
Then 18 g of KMnO4 was slowly added.
N-methyl imidazole (27.0 g) was slowly added while being stirred under an ice bath.
Afterwards, 9 g of KMnO4 was slowly added in the solution.
N-methyl imidazole (27.0 g) was slowly added with stirring under ice bath.
Then, 1.26 g TIP was slowly added to the solution and stirred for 30 min.
Saturated ammonium sulfate (30%) was slowly added to the crude extract with constant stirring.
After the solution changed from gray to yellow, a quantity of cobaltous dihydroxycarbonate was slowly added.
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The hypotonic solution was slowly added to a volume of 2 ml.
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