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The extract was shaken using a vortex mixer for 30 s and centrifuged at 10,000 rpm for 5 min. 2 mL of the supernatant was used to analyze the presence of CPyF and its degraded metabolites using LC MS as mentioned in analytical methods.
The tube was shaken using a tube mixer for 10 min, and the absorbance was measured.
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The bottles were shaken using a mechanical shaker for 15 min.
Harvesting tests were also conducted using shaking frequencies of 14 and 18 Hz, and test trees were shaken using a sequence of four 5 s long intermittent excitations.
The solutions were shaken using a shaker, filtered, and the fibers were dried under a gentle flow of nitrogen for 2 min, prior to desorption studies.
Samples were shaken using the Genogrinder for 3 min at a setting of 500 strokes min−1.
Definity Perflutren lipid microspheres (Lantheus Medical Imaging, North Billerica, MA, USA) were shaken using a Lantheus device for 45 s at 3000 r.p.m.
The tube was submerged in liquid nitrogen, after which it was vigorously shaken using a vortex shaker to separate extremities from the body.
The mixture was then shaken using a vortex for 1 min and left for about 20 min in a water bath (temperature 50°C to 55°C) until it was separated into two phases.
Prior to the analysis, each sample was shaken vigorously using open-air platform shakers to achieve homogeneity.
The tube was shaken by using a 'head-over-head' shaker.
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