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Then the library pool was sequenced in one GS FLX Titanium run on 1/8th of a 70 × 75 PicoTiter plate.
Pooled DNA from parents, 189 RILs, 94 other lines and 3 blanks was sequenced in one lane with the Illumina HiSeq sequencer at Genomic Diversity Facility, Cornell University Institute of Biotechnology (http://www.biotech.cornell.edu/brc/genomic-diversity-facility).
Each sample was sequenced in one lane of an Illumina Genome Analyzer IIx sequencer.
Each library was sequenced in one lane.
Each library was sequenced in one region of the PicoTiterPlate.
Salinas library was sequenced in one direction while the L. serriola acc.
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Each pool of twelve samples was sequenced in one-sixteenth of a 70 × 75 PicoTiterPlate.
Libraries prepared from the 95 samples and one blank negative control were sequenced in one lane on an Illumina HiSeq 2500 (Illumina, San Diego, California, USA).
Three biological replicate RNA samples from each stage of the leaves were sequenced in one lane on the Illumina Hi-Seq 2500, and the number of reads ranged from 5,000,000 to 18,000,000.
In that case, barcoding power could be increased by using both barcoded forward and reverse PCR primers, but this would restrict the length of the sequenced regions, as each region would only be sequenced in one direction.
Clearly large crop collections cannot be sequenced in one draft.
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