Sentence examples for was sequenced directly from inspiring English sources

Each gene amplicon was sequenced directly.

The oprD gene of isolate Be128 was sequenced directly from genomic DNA.

DNA was sequenced directly, with fragments amplified by the dideoxy chain-termination method from the strains described above.

If a single amplification product was obtained, the PCR product was sequenced directly using the amplification primers.

For sequencing, 10 µl of the PCR products was treated with 10 U of exonuclease I and 1 U of shrimp alkaline phosphatase (United States Biochemical, USA) at 37°C/15 min, followed by 15 min incubation at 80°C. 4 µl of treated PCR product was sequenced directly or following cloning into pGEM-T Easy vector (Promega, USA) using specific primers and an ABI 3730 capillary sequencer (Applied Biosystems).

The PCR product was sequenced directly using the forward primer.

Gel-extracted PCR products were sequenced directly on a 3730 96-capillary sequencer (Applied Biosystems).

Both sense and antisense strands were sequenced directly on an Applied Biosystems 377 DNA sequencer using BigDye terminator cycle sequencing.

Amplification products were sequenced directly using ABI BigDye 3.1 and the ABI 3100 automated sequencer.

So only a small part of the genome has to be sequenced directly.

These PCR fragments can be sequenced directly without the need for cloning PCR fragments first.