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Approximately 2 kb of the divergent region was sequence to complete the 5' end of the 12S rRNA gene and its analysis was not included in this thesis.
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Finally, the clone was sequenced to confirm correct insertion.
Plasmid DNA was sequenced to identify transformants of each allele.
The mutated fragment was sequenced to confirm the desired mutation.
The NA gene was sequenced to subtype the viruses.
The construct was sequenced to verify frame.
The transgene was sequenced to ensure authenticity of the sequence.
from South Carolina [ 12], was sequenced to 120-fold coverage.
The plasmid was sequenced to confirm the point mutation.
The constructed plasmid was sequenced to confirm identity.
The new vector was sequenced to ensure correct insertion.
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