The first issue, "not to be sold separately", contained prominent pictures by John Hedgecoe (including the Mountbatten portrait), Loomis Dean, and a photo story on the London stock exchange by Gerry Cranham.
Whole blood was separately collected in heparin-containing tubes precoated with mitogen as a positive control or cocktails of ESAT-6, CFP-10, and TB7.7 (peptidesides as Mtb-specific antigens (QuantiFERON®-TB Gold in Tube; Cellestis); the negative control tubes had no precoat.
For measuring APX activity, the tissue was separately ground in homogenizing medium containing 2.0 mM AsA in addition to the other ingredients.
The phase containing the granulocytes was separately transferred to a new tube and washed with PBS.
Kinetic studies, 'in situ' characterizations and H/D isotopic exchange experiments were separately performed on catalysts containing small Ru nanoparticles and catalysts containing larger particles, in order to elucidate the mechanism of catalytic cooperation.
To achieve this objective, twenty-one dogs with epilepsy were enroled into a 6-month prospective study, in which they were separately fed the MCTD containing low concentrations of MCT (5·5 % as fed) for 3 months and an isoenergetic placebo diet containing the same levels of fat, protein and carbohydrate for 3 months.
Microcrystalline cellulose (MCC) and polyamide 66 (PA66) have been separately dissolved in solutions containing trifluoroacetic acid (TFA) as a common solvent, mixed after their dissolution and cast.
Nine micrograms of each plasmid vector were separately added to tubes containing Opti-MEM-I Opti-MEM-I Opti-MEM-Ind FuGENE 6 transfection reagent (Roche, Basel, Switzerland); each plasmediuns then separately transfected Invitrogenm dishes contanding Platinum-E packaging cells [38].
The wild-type strain GD201008-001, mutant strain Δ xf and the complementation strain CΔ xf were separately inoculated into flasks containing 100 mL TSB media, and incubated at 37°C and 28°C.
The gene sequences (Table 2) were used to design PCR primers and/or overgo probes for each gene (Additional file 2, Tables S1 and S2), and macroarrays were separately screened with a pool containing the radiolabeled overgos and a pool containing the radiolabeled PCR amplicons.
