Sentence examples for was separated onto from inspiring English sources

After 41 h incubation the trophozoite-enriched fraction was separated onto and collected from a discontinuous 10/40/80% Percoll gradient.

Stratum corneum extract was mixed with 4x NuPAGE LDS Sample Buffer (Invitrogen) and 0.5 µg of this extract was separated onto a NuPAGE 4-12% Bis-Tris Gel (Invitrogen).

The 314 bp PCR product was separated onto a 1% agarose gel and was purified with a Gel purification Kit (Qiagen, Valencia, CA).

Total proteins were quantified using the Pierce BCA Protein Assay Kit Thermo Scientificc, Rockford, IL, USA), and 30 μg of protein per sample was separated onto a denaturing polyacrylamide gel containing SDS, and then transferred to a methanol-activated PVDF filter membrane (Bio-Rad, Hercules, CA, USA).

About 200 µg of the total pooled RNAs were separated onto a denaturing 15% polyacrylamide gel.

Total proteins were separated onto SDS-PAGE gels, which were dried and exposed to X-ray films.

Fifty micrograms of total cellular protein were separated onto 12% Nupage gels Tris-HCl sodium dodecyl sulfate (SDS -PAGE, tranSDS -PAGEntransferredlontoVDF membranes, and blocked with 5% natural nonfat dry milk as described before.

Equal amounts of total proteins (40 µg) determined by the Bradford method (BioRad, Marnes-La-Coquette, France) were separated onto 12% SDS-PAGE then transferred on nitrocellulose membrane (Schleicher & Schuell, Dassel, Germany).

For this, the three cytoplasmic rRNA from both cell lines were separated onto an agarose gel, extracted from the gel and submitted to methylation analysis according to the method described in Fig. 4.

Samples were separated onto 10% SDS-PAGE.

PCR products were separated onto agarose gels.