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Sleep was scored using both standard clinical metrics and power spectral analysis.
Gait was scored using a 6-point scale at the end of gestation.
The degree of osteoarthritis was scored using Kellgren and Lawrence scale [11].
Pathological tumour regression grade was scored using American Joint Committee on Cancer AJCCC) 7.0.
Contrast resolution was scored using a 10-point scale at three levels: liver, spleen and gluteus maximus.
Desire was scored using an established model called the Female Sexual Function Index, which ranges from 1.2 to 6.0.
Immunostaining was scored using three semi-quantitative IHC scoring systems.
Overlap was scored using Fisher's Exact Test p-value.
GFP fluorescence was scored using an Axioplan2 fluorescence microscope (n = 100 ASH neurons).
All experiments were videotaped and behavior was scored using Jwatcher [20].
TUNEL staining was scored using the 100x optic on a Zeiss Axioplan 2 microscope.
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