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Synechococcus sp. PCC 7002 was reported to require vitamin B12 (cobalamin) for in vitro growth (Wilhelm and Trick 1995) and it was confirmed in our study (Fig. 2).
Flagellin-independent caspase-1 activation by S. Typhimurium was reported to require increased multiplicities of infection.
This contrasts with previous findings from one study of mouse iNKT cells, for which IFNγ production was reported to require a stronger TCR activation signal than IL-4 as a result of a signaling mechanism involving c-rel expression [24].
The mechanism of Syk activation by αIIbβ3 integrin was reported to require the ITAM-containing FcγIIA molecule [ 26].
These results differ from daunorubicin induction of nSMase2 in MCF7 cells, which was reported to require the transcription factors Sp1 and Sp3 – which appear to be dispensable in our system.
Recently, Wnt signaling was reported to require endocytosis (Bellen & Seto 2006; Blitzer & Nusse 2006; Yamamoto et al. 2006), and Wnt proteins outside the cell were proposed to be internalized via endocytosis and function in the endosome (Niehrs & Boutros 2010).
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His one remaining sister is also physically handicapped and is reported to require constant care.
The B.I.G. is reported to require about 17 s for preparation and insertion [26].
The system is reported to require 100-fold less sample volume, tenfold less buffer volume and lower electric fields than conventional FFE systems.
In contrast to MDCK cells, G-402 cells are reported to require 160-times more ε-toxin to kill than MDCK cells [39].
Although most cysteine proteases have not been reported to require oligomerization for catalysis, crystallographic data showed homodimerization for another USP, USP8 [28], providing further grounds for USP25 dimerization.
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