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After 20 h of cultivation in a microtiter plate shaker (Multitron II, Infors GmbH, 37 °C, 900 rpm, 70%% humidity), each well was replicated by using a replicator (EnzyScreen BV, Leiden, Netherlands) into a second series of 96-well microtiter plates containing 150 μl of induction medium MD-5052 [ 41] supplemented with kanamycin (60 μg/ml).
A lung tumour was replicated by using a ball of wax placed in the lung close to the heart.
Prior image analysis [16] was replicated by using Matlab and Statistical Parametric Mapping software (SPM5; Wellcome Department of Cognitive Neurology, http://www.fil.ion.ucl.ac.uk/spm).ac.uk/spm
The same results were obtained when the experiment was replicated by using a RPM to model the microgravity conditions [ 12].
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Notably, previously reported dose-dependent changes in tenocyte bioactivity to soluble IGF-1 within the CG scaffold were replicated by using a single dose of soluble IGF-1 in scaffolds containing increasingly sulfated GAGs.
The deletion library was replicated by inoculation into 96-well plates using a BioMek liquid-handling robot (Perkin Elmer) and grown in 150 μl YPD media (1% yeast extract, 2% bacto peptone, 2% glucose) to saturation at 30° for 48 hr.
This general finding has been replicated by other psychologists using a variety of disgust elicitors and moral behaviors.
Indeed, we were the first to describe a subordination-induced weight gain in mice during the chronic psychosocial stress procedure [26], a finding that has now been replicated by other groups using similar preclinical animal models of social stress [27] [30], [40].
This will reduce the risk of errors (which could be replicated by other researchers using these data).
Further, as a novel bio-mimetic approach for tribological application at micro-scale, the surface topography of natural leaves of Lotus and Colocasia were replicated by capillary force lithography using two different molding techniques.
Most psychometric analyses were replicated by a second statistician using SAS 8.2.
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