In the experiments in which all 23 cysteines were replaced individually, we observed that most of the cysteine replacements were tolerated by the protein, but the replacement of C1056, C1091, C1106, C1294 or C1336 resulted in an enhanced drug susceptibility together with an abrogated drug efflux.
Many years after the events presently being recounted, at a dinner in our heroine's own house, a philosopher sitting to her right — quoting another philosopher — suggested that she undertake a thought experiment: What if your brain cells were replaced individually with the brain cells of another person?
This is a six-color printer, and each color ink cartridge can be replaced individually — so you don't need to worry about wasting money should you just run out of cyan.
"If you break one of [the] buttons, it can be replaced individually from the others; however, you still need to get inside the watch in the first place, a feat we have not yet mastered," they write.
To evaluate the importance of tyrosine residues in PTB binding, we synthesized peptide analogs in which the triad Tyr residues were replaced, individually or in combination, by Ala, Phe or pTyr residues.
They also reduce waste because if one is damaged it can be replaced individually instead of replacing the entire patio.
After 24 h, DMEM was replaced with FAD medium and oesophageal cells were sorted individually in each well.
To define the function of the ZNF motif in pUL31, cysteines C73, C89, and C92 were individually replaced by serines, and the histidine H188 was replaced by an alanine, and functional analyses of the mutant proteins were performed both in vitro with artificial membranes and in situ in eukaryotic cells.
